Oxidative stress and expression level of Catalase, Glutathione S Tranferase Enzyme in type 2 diabetes

Type2 diabetes formerly known as non insulin dependent diabetes is the most common form of hyperglycemia, insulin resistance and relative insulin deficiency. The type 2 diabetes is caused due to metabolical disorder. Oxidative stress, through production of reactive oxygen species (ROS) to develop insulin resistance, beta cell dysfunction, impaired glucose tolerance there by impairing antioxidant expression level such as catalase.Type2 diabetes is develop by oxidative stress due to imbalance in antioxidant expression and activity. Peroxisome proliferators’ activated receptor (PPAR) is able for catalase enzyme development. Reactive molecule species are bind with PPAR and inhibit to signalling pathways which initial for activity of catalase enzyme due in that condition decrease level of catalase activity so that catalase can not convert to lot of reactive molecule in H2O and O2. The data are suggested that PPAR mediated receptor signalling pathway and expression of gene may block to normal catalase expression which by reactive molecules species. In study this we have to measure Red Blood Cell catalase (CAT), Total Antioxidant Level (TAC), Glutathione S Transferase (GST) to understand weather what kind of Enzyme activity in Type2 Diabetes Patients and in Healthy person male or female. Method and Result We measure activity of CAT enzyme in Diabetes patients (N=60) and Healthy individual (N=30) Mean and SEM value of CAT enzyme in Diabetes & Healthy Individual respectively 0.6283 ± 0.1209 and 0.9754±0.1787. GST in Diabetes & Healthy Individual respectively 61.70 ± 9.123 and 55.52 ± 8.094.TAC level of Diabetes & Healthy Individual respectively 17.41 ± 0.8497 and 39.58 ± 2.061.